Effect of laminin, polylysine and cell medium components on the attachment of human hepatocellular carcinoma cells to cellulose nanofibrils analyzed by surface plasmon resonance

The development of in vitro cell models that mimic behavior organs and tissues is an approach may have remarkable impact on drug testing tissue engineering applications the future. Plant-based, chemically unmodified cellulose nanofibrils (CNF) hydrogel a natural, abundant, biocompatible material has attracted great attention for biomedical applications, particular three-dimensional cultures. However, mechanisms cell-CNF interactions factors affect these are not yet fully understood. In this work, multi-parametric surface plasmon resonance (SPR) was used to study how adsorption human hepatocellular carcinoma (HepG2) cells CNF films affected by different proteins components medium. Both recombinant laminin-521 (LN-521, natural protein extracellular matrix) poly-l-lysine (PLL) adsorbed enhanced attachment HepG2 cells. Cell medium (glucose amino acids) serum (fetal bovine serum, FBS) also both bare protein-coated substrates. FBS hindered LN-521- PLL-coated substrates, suggesting blocked formation laminin-integrin bonds decreased favorable PLL-cell electrostatic interactions. This work sheds light effect CNF, paving way utilization optimization CNF-based materials applications.